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PC versions of this product are sold exclusively from the internet. You will receive the software. Krista Leigh Steinke, Filmmaker, is a lens-based artist and educator working in photography, moving image, and installation. Her work, situated between the photographic and the abstract, presents poetic reflections on time, place, perception, and the interconnection between human experience and the natural world. She regularly exhibits and screens her work in museums, galleries, and film festivals across the country, as well as internationally.

He is a former basketball journalist and Editor for Ugly Duckling Presse. We uphold diverse minority voices, layer multiple genres, encourage collaborative possibilities, and provide new opportunities for poets We pay poets for their work and initiate collaborative partnerships that enrich the cultural community, delivering poetry to audiences in Houston and beyond. Public Poetry is committed to inclusivity and diversity as a core value that determines how we operate as an organization; how we develop and implement our programs and projects; and how we activate our community outreach.

We actively strive to create a social ecosystem where mutual respect thrives; where cultural and physical differences are acknowledged and enabled; and where social justice and anti-racist values are manifest.

Cassells is the recipient of a Pushcart Prize, the Peter I. He has worked as a translator, film critic, actor and teacher. He is a professor of English at Texas State. Hardison, Jr. He lives in Santa Fe, New Mexico. In , her third book, sombra: dis locate and a chapbook, profeta without refuge, were released. She is currently an associate professor of education in the Kalmanovitz School of Education at St.

Her choose-your-own adventure love story Old Cat Lady is forthcoming from Books in October Join Mary McDonald, award winning media artist for a live talk with Q and A on the possibilities offered by Augmented and Mixed Reality, and binaural sound, and mixed media layers. Learn tools, techniques, tips and tricks for going beyond flat video to create immersive artistic experiences.

Explore the ways to expand the boundaries of your art. McDonald will demonstrate the tools and techniques used to create her three most recent projects — Times in Sound , a Binaural Sound experience, Augmented Reality poetry installation, On the Margin of History and the multilayered poetry film, Wishing Well, for River Revery, an outdoor Augmented Reality poetry installation.

Join award winning media artist and poetry filmmaker Mary McDonald for a hands-on workshop on how to use accessible mobile editing tools for video and photos. These mobile apps allow you to easily perform sophisticated editing of film and photos, opening the door to all poets and artists, no experience necessary.

Bring your smartphone or tablet, photos and video clips, and your unique vision and creativity. To participate in this hands-on workshop, please download the mobile video editing app prior to the workshop. Videoleap iPhone or Vivacut Android. For two years, Helen Dewbery has been collaborating with poets published by Nine Arches Press to create poetry film. Showing some of these poetry films, Helen will talk about how she approaches this work, the challenges that have arisen, and some of the key processes used to create these unique works.

Helen Dewbery talks about, writes about and creates poetry films, and established Poetry Film Live, the online poetry film journal. Her poetry films have been shown at poetry events and festivals in the UK and internationally. She provides training and support for new practitioners of poetry film and has worked with poets to make collaborative work. Stone , []. Lost: Ann McCrady ] 3.

Using deaf sign language, she injects a compelling element that animates her work and speaks to us in new ways. Sabina is including a new videopoem in progress that will premier at REELpoetry, and has also curated this selection of films that samples some of her work. Sabina England is a filmmaker, playwright and performance artist.

She studied at London Film Academy and received a certificate in filmmaking. She then pivoted to filmmaking, releasing silent comedy shorts on Youtube, which got the attention of famed movie critic, Roger Ebert, who praised her works in one of his newsletters. Sabina then wrote, directed and produced her first narrative short, Wedding Night, which premiered at Tribeca Cinemas in New York City. Over the years, Sabina has won several awards for her short films and multimedia projects.

You may visit SabinaEngland. So, how do we incorporate virtual elements into live festivals as we go forward? Join panelists Chaucer Cameron, Helen Dewbery, Sabina England, Lucy English; Eleanor Livingstone, Mary McDonald, Fran Sanders and Sarah Tremlett as they think out loud, speculate and conjecture, explore and experiment, and wrestle with their collective thoughts of this hybrid hydra that becomes a new form of festival with infinite possibilities. Wild Whispers is an exciting and collaborative transnational poetry film project.

It started with one poem and led to 14 versions in 10 languages and 12 poetry films. Chaucer will talk about the inspiration behind this project and show some of the films. Her poetry has also been published in journals, anthologies and online. Her poetry-films have screened at festivals, universities and poetry events. Chaucer is co-editor of P oetry Film Live.

A ground-breaking industry bible for anyone interested in poetry, digital media, filmmaking, art and creative writing as well as poetry film-makers. She has given presentations on her own poetry films worldwide. Bio — Helen Dewbery UK talks about, writes about and creates poetry films, and established Poetry Film Live, the online poetry film journal.

Her first full collection, Even the Sea was shortlisted for the inaugural London New Poetry award and is now in a second edition. Lucy is a spoken word poet and novelist. K and Athens, Greece. Bio — Mary McDonald Canada pursues multidisciplinary practices encompassing text, photography, poetryfilm, music and sound, interactive AR augmented reality installations, and community participatory arts projects.

Please try to purchase from your local independent bookseller before you shop on Amazon. Mister Bubbles from Boxed series. However, his body is flaccid and bulbously round, a laughable empty plastic shell with air bubbles in his groin, and no guts. Bubbles is obviously full of hot air, with bubbles that can be easily popped.

Since launching in April , we have been in 33 different venues, from inside the loop to beyond the beltway, organized poetry events, with poets participating, including a Poet Laureate of the United States, 4 Texas State Poet Laureates and 2 Pulitzer Prize nominees together with local, regional, nationally and internationally recognized poets. To review programs by year click HERE. After you have registered, please email us at Publicpoetryon gmail.

When we have both your registration and your follow up email, you will receive a confirmation email from us. Like a supermarket chicken, a brain weighs about three pounds, without feathers of course. Federal government websites often end in. The site is secure. The importance of further elucidating the properties surrounding microchimerism in various experi- mental models and clinical transplantation are limited by current techniques and the sensitivity of available platforms.

Development of reliable methods and use routine use of microchimerism detection in clinical practice could guide clinical decision making regarding rejection, stable function, and tolerance. The path toward immunologic tolerance in organ transplantation has the most promise via strategies that achieve either transient or durable chimerism. Nonetheless, the immunologic consequence of microchimerism are largely unknown.

The genetic closeness of this island population results in only 7 distinct MHC haplotypes that affect methods available to differentiate donor from recipient, and correspondingly chimerism. This is compared to the identified class I and class II MHC sequences that continues to grow in numbers, isolated from rhesus, cynomolgus, and pig-tailed macaques commonly used in research.

Our group and others who work with the MCM model have generally used cross-reactive anti-human leukocyte antigen HLA antibodies to identify selective class I mismatching at the major histocompatibility complex MHC. Post-transplant, anti-HLA antibodies are used to detect and follow for any development of chimerism. The use of PCR techniques and next generation sequencing as an alternative to antibody chimerism detection methods in NHP could allow for more sensitive quantitative assays.

Next generation sequencing, utilizing technology based in PCR, allows for not only the quantification of genes but also gives sequence information. These techniques applied to donor and recipient genetic differences are the basis for chimerism assays. As a result, a non-specific primer targeting a conserved sequence across all haplotypes based on MAFA alleles, which are HLA equivalents, should allow for the amplification and quantification of specific donor sequences to quantify chimerism.

Primers successfully amplified by standard PCR a region of Mafa-B with known variability in sequence respective to haplotype. The product of interest was confirmed in length via gel electrophoresis, and melting curves demonstrated that the region of interest was only amplified signifying single, specific products. When sequencing these different products after PCR, the number of alleles of each haplotype would be identified.

The number of sequences for each corresponding haplotype should have allowed for quantification of chimerism. When known haplotype animals were tested sequencing found that appropriate PCR products were amplified for all animals; however, presumably due to differences in efficiency not all alleles of each haplotype amplified at the same rate. Thus, sequenced ratios did not represent starting DNA ratios. Haplotype specific primers could be constructed as a next step; however, equal and somewhat specific amplification of multiple primers across multiple haplotypes would be necessary for accurate quantification.

While one primer may not work for all scenarios, differentiation between the most common haplotypes would be progress toward PCR-based chimerism assays. Some of the clinical and immunologic rationale to select MCM for transplantation studies also result in unique challenges in chimerism detection. The use of PCR and Ion Torrent sequencing should ultimately be a more efficient way to detect microchimerism in primate models.

Currently, the development of non-specific primers to amplify all alleles with high PCR efficiencies is a challenge. Moving forward, the design of the primer is critical and the basis to the success of chimerism detection using this method. Focus is now on optimizing PCR conditions to provide equal and optimized yields.

We have considered combining primers to ensure all alleles are amplified. Multiple primers could be given the same barcode making it possible to identify differences between alleles, while knowing that they came from the same sample. However, all options should be explored with a non-specific primer set. Using one general non-specific primer to amplify all haplotypes would allow for a much more simplistic assay without the need to mix multiple primers before PCR.

The use of sensitive methods for detecting chimerism levels is of interest in solid organ transplantation. In kidney transplantation tolerance trials, various methods have been used to quantitate chimerism. In general, flow cytometry cell sorting is performed followed by short tandem repeat STR analysis.

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